Real-time quantitative PCR assay on bacterial DNA: In a model soil system and environmental samples.

Abstract

Real-time quantitative PCR (RTQ-PCR) was used to quantify the bacterial target DNA extracted by three commonly used DNA extraction protocols (bead mill homogenization, grinding in presence of liquid nitrogen and hot detergent SDS based enzymatic lysis). For the purpose of our study, pure culture of Bacillus cereus (model organism), sterilized soil seeded… (More)

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@article{Kabir2003RealtimeQP, title={Real-time quantitative PCR assay on bacterial DNA: In a model soil system and environmental samples.}, author={Shaila Kabir and Narasimmalu Rajendran and Takashi Amemiya and Kiminori Itoh}, journal={The Journal of general and applied microbiology}, year={2003}, volume={49 2}, pages={101-9} }