Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi

@article{Schena2004RealtimeQP,
  title={Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi},
  author={Leonardo Schena and Franco Nigro and Antonio Massimo Ippolito and Donato Gallitelli},
  journal={European Journal of Plant Pathology},
  year={2004},
  volume={110},
  pages={893-908}
}
Real-time PCR technologies open increasing opportunities to detect and study phytopathogenic and antagonistic fungi. They combine the sensitivity of conventional PCR with the generation of a specific fluorescent signal providing real-time analysis of the reaction kinetics and allowing quantification of specific DNA targets. Four main chemistries are currently used for the application of this technique in plant pathology. These chemistries can be grouped into amplicon sequence non-specific (SYBR… CONTINUE READING

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