Real-time quantitative PCR, pathogen detection and MIQE.

@article{Johnson2013RealtimeQP,
  title={Real-time quantitative PCR, pathogen detection and MIQE.},
  author={Gemma L. Johnson and Tania Nolan and Stephen Bustin},
  journal={Methods in molecular biology},
  year={2013},
  volume={943},
  pages={1-16}
}
Nucleic acids are the ultimate biomarker and real-time PCR (qPCR) is firmly established as the method of choice for nucleic acid detection. Together, they allow the accurate, sensitive and specific identification of pathogens, and the use of qPCR has become routine in diagnostic laboratories. The reliability of qPCR-based assays relies on a combination of optimal sample selection, assay design and validation as well as appropriate data analysis and the "Minimal Information for the Publication… CONTINUE READING
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