Real-time polymerase chain reaction microRNA detection based on enzymatic stem-loop probes ligation.

Abstract

MiRNAs (microRNAs) are a group of endogenous, small noncoding RNA with the length of 18-25 nucleotides, which have recently been demonstrated to play important roles in a wide range of biological processes. In this work, we developed a simple, sensitive, specific, and inexpensive assay through the combination of enzymatic probe ligation and real-time PCR… (More)
DOI: 10.1021/ac900598d

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