Real-time polymerase chain reaction in bronchial aspirate for rapid detection of sputum smear-negative tuberculosis.

@article{Min2010RealtimePC,
  title={Real-time polymerase chain reaction in bronchial aspirate for rapid detection of sputum smear-negative tuberculosis.},
  author={J Min and Ho Il Yoon and Kyoung Un Park and J Song and Chien-Te Lee and J H Lee},
  journal={The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease},
  year={2010},
  volume={14 7},
  pages={852-8}
}
SETTING The real-time polymerase chain reaction (RT-PCR) has increasingly been used for the detection of various micro-organisms, including mycobacteria. OBJECTIVE To determine the role of RT-PCR in confirming the diagnosis of tuberculosis (TB) when acid-fast bacilli (AFB) smear results in sputum samples were not available (i.e., no sputum or negative smear results). DESIGN We analysed the data of consecutive patients whose bronchial aspirate (BA) was tested for RT-PCR for the diagnosis of… CONTINUE READING
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