Real-time polymerase chain reaction detection of Neisseria meningitidis in formalin-fixed tissues from sudden deaths.

@article{FernndezRodrguez2008RealtimePC,
  title={Real-time polymerase chain reaction detection of Neisseria meningitidis in formalin-fixed tissues from sudden deaths.},
  author={Amparo Fern{\'a}ndez-Rodr{\'i}guez and Bel{\'e}n Alcal{\'a} and Roverto Alvarez-Lafuente},
  journal={Diagnostic microbiology and infectious disease},
  year={2008},
  volume={60 4},
  pages={339-46}
}
Accurate identification of meningococcal sudden deaths is needed to avoid underestimation of the true incidence of the disease. This study analyzed the usefulness of a real-time polymerase chain reaction (PCR) protocol using MGB (3'-minor groove binder) probes to detect Neisseria meningitidis in formalin-fixed paraffin-embedded tissues from sudden deaths where a meningococcal fulminating infection was suspected. The protocol included detection of meningococcal DNA (ctrA gene), multiplex B/C PCR… CONTINUE READING