Real-time polymerase chain reaction: a novel molecular diagnostic tool for equine infectious diseases.

@article{Pusterla2006RealtimePC,
  title={Real-time polymerase chain reaction: a novel molecular diagnostic tool for equine infectious diseases.},
  author={Nicola Pusterla and John E. Madigan and Christian M. Leutenegger},
  journal={Journal of veterinary internal medicine},
  year={2006},
  volume={20 1},
  pages={
          3-12
        }
}
The focus of rapid diagnosis of infectious disease of horses in the last decade has shifted from the conventional laboratory techniques of antigen detection, microscopy, and culture to molecular diagnosis of infectious agents. Equine practitioners must be able to interpret the use, limitations, and results of molecular diagnostic techniques, as they are increasingly integrated into routine microbiology laboratory protocols. Polymerase chain reaction (PCR) is the best-known and most successfully… CONTINUE READING

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