Real-time observation of signal recognition particle binding to actively translating ribosomes

@inproceedings{Noriega2014RealtimeOO,
  title={Real-time observation of signal recognition particle binding to actively translating ribosomes},
  author={Thomas R. Noriega and Jin Chen and Peter J. Walter and Joseph D. Puglisi},
  booktitle={eLife},
  year={2014}
}
The signal recognition particle (SRP) directs translating ribosome-nascent chain complexes (RNCs) that display a signal sequence to protein translocation channels in target membranes. All previous work on the initial step of the targeting reaction, when SRP binds to RNCs, used stalled and non-translating RNCs. This meant that an important dimension of the co-translational process remained unstudied. We apply single-molecule fluorescence measurements to observe directly and in real-time E. coli… CONTINUE READING

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