Real-time observation of affinity reactions using grating couplers: determination of the detection limit and calculation of kinetic rate constants.

Abstract

The use of integrated optical grating couplers for the analysis of bioaffinity reactions in order to calculate kinetic rate constants was investigated. The specificity of the sensor surface was determined by adsorptive or covalent attachment of the specific ligands. As an evanescent field sensor, the specific interaction of the corresponding ligand could be… (More)

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