Real-time observation of DNA translocation by the type I restriction modification enzyme EcoR124I

@article{Seidel2004RealtimeOO,
  title={Real-time observation of DNA translocation by the type I restriction modification enzyme EcoR124I},
  author={Ralf Seidel and John van Noort and Carsten van der Scheer and Joost G. P. Bloom and Nynke H Dekker and Christina F. Dutta and Alex Blundell and Terence Robinson and Keith Firman and Cees Dekker},
  journal={Nature Structural &Molecular Biology},
  year={2004},
  volume={11},
  pages={838-843}
}
Type I restriction enzymes bind sequence-specifically to unmodified DNA and subsequently pull the adjacent DNA toward themselves. Cleavage then occurs remotely from the recognition site. The mechanism by which these members of the superfamily 2 (SF2) of helicases translocate DNA is largely unknown. We report the first single-molecule study of DNA translocation by the type I restriction enzyme EcoR124I. Mechanochemical parameters such as the translocation rate and processivity, and their… CONTINUE READING

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