Real-time observation of DNA looping dynamics of Type IIE restriction enzymes NaeI and NarI

@article{Broek2006RealtimeOO,
  title={Real-time observation of DNA looping dynamics of Type IIE restriction enzymes NaeI and NarI},
  author={Bram van den Broek and Francesco Vanzi and Davide Normanno and Francesco Pavone and Gijs J. L. Wuite},
  journal={Nucleic Acids Research},
  year={2006},
  volume={34},
  pages={167 - 174}
}
Many restriction enzymes require binding of two copies of a recognition sequence for DNA cleavage, thereby introducing a loop in the DNA. We investigated looping dynamics of Type IIE restriction enzymes NaeI and NarI by tracking the Brownian motion of single tethered DNA molecules. DNA containing two endonuclease recognition sites spaced a few 100 bp apart connect small polystyrene beads to a glass surface. The position of a bead is tracked through video microscopy. Protein-mediated looping and… CONTINUE READING
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