Real time monitoring of sickle cell hemoglobin fiber formation by UV resonance Raman spectroscopy.


In sickle cell hemoglobin, individual tetramers associate into long fibers as a consequence of the mutation at the beta6 position. In this study UV resonance Raman spectroscopy is used to monitor the formation of Hb S fibers in real time through aromatic amino acid vibrational modes. The intermolecular contact formed by the mutation site ((1)beta(1)6 Glu… (More)
DOI: 10.1021/bi901352m