Real-time monitoring of dynamic intracellular Ca(2+) movement during early embryogenesis through expression of yellow cameleon.

@article{Tsuruwaka2007RealtimeMO,
  title={Real-time monitoring of dynamic intracellular Ca(2+) movement during early embryogenesis through expression of yellow cameleon.},
  author={Yusuke Tsuruwaka and Takafumi Konishi and Atsushi Miyawaki and Masahiro Takagi},
  journal={Zebrafish},
  year={2007},
  volume={4 4},
  pages={253-60}
}
Intracellular calcium ion (Ca2+) is important in a variety of developmental events in the vertebrate embryo through wide-ranging signal transduction pathways. The purpose of this study was to detect dynamic changes in Ca2+ during gastrulation using yellow cameleon 2.12 (YC2.12), a Ca2+ indicator protein based on cyan and yellow fluorescent proteins. mRNA for YC2.12 was synthesized in vitro, and injected into early embryos at the single-cell stage. Fluorescence images were obtained using a… CONTINUE READING

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