Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen.

Abstract

This paper describes the cultivation of primary cells in a microchamber and the real-time monitoring of small amounts of antibody secretion after the introduction of a minute amount of stimulus by a microinjector using time-resolved fluorescence anisotropy analysis.

Cite this paper

@article{Munaka2007RealtimeMO, title={Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen.}, author={Tatsuya Munaka and Hirohisa Abe and Masaki Kanai and Takashi Sakamoto and Hiroaki Nakanishi and Shuichi Shoji and Shinya Kimura and Taira Maekawa and Akira Murakami}, journal={The Analyst}, year={2007}, volume={132 6}, pages={512-4} }