Real time monitoring of acylations during solid phase peptide synthesis: a method based on electrochemical detection.

@article{Nielsen1989RealTM,
  title={Real time monitoring of acylations during solid phase peptide synthesis: a method based on electrochemical detection.},
  author={C S Nielsen and Per Hertz Hansen and A. Lihme and Peter Michael Heegaard},
  journal={Journal of biochemical and biophysical methods},
  year={1989},
  volume={20 1},
  pages={69-79}
}
Monitoring of acylation reactions during solid phase peptide synthesis is important to ensure high coupling yields in all steps of the synthesis. We describe in this paper a simple and reliable method for monitoring the time course of the acylation steps as well as the washing and deprotection steps during computer-controlled solid phase peptide synthesis… CONTINUE READING