Real-time measurements of amino acid and protein hydroperoxides using coumarin boronic acid.

Abstract

Hydroperoxides of amino acid and amino acid residues (tyrosine, cysteine, tryptophan, and histidine) in proteins are formed during oxidative modification induced by reactive oxygen species. Amino acid hydroperoxides are unstable intermediates that can further propagate oxidative damage in proteins. The existing assays (oxidation of ferrous cation and… (More)
DOI: 10.1074/jbc.M114.553727

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