Real-time measurement of endosomal acidification by a novel genetically encoded biosensor.

  title={Real-time measurement of endosomal acidification by a novel genetically encoded biosensor.},
  author={Michela Serresi and Ranieri Bizzarri and Francesco Cardarelli and Fabio Beltram},
  journal={Analytical and bioanalytical chemistry},
  volume={393 4},
Genetically encoded fluorescent proteins are optimal reporters when used to monitor cellular processes as they can be targeted to any subcellular region by fusion to a protein of interest. Here, we present the pH-sensitive fluorescent protein E(1)GFP which is ideally suited to monitor pH changes in dynamic intracellular structures in real time with high spatio temporal resolution. E(1)GFP is a ratiometric pH indicator by emission with a pK close to 6.0. We describe an application of this novel… CONTINUE READING

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