Corpus ID: 235794893

# Real time large scale $\textit{in vivo}$ observations by light-sheet microscopy reveal intrinsic synchrony, plasticity and growth cone dynamics of midline crossing axons at the ventral floor plate of the zebrafish spinal cord

@inproceedings{Andersen2021RealTL,
title={Real time large scale \$\textit\{in vivo\}\$ observations by light-sheet microscopy reveal intrinsic synchrony, plasticity and growth cone dynamics of midline crossing axons at the ventral floor plate of the zebrafish spinal cord},
author={S{\o}ren S L Andersen},
year={2021}
}
Axonal growth and guidance at the ventral floor plate is here followed in vivo in real time at high resolution by light-sheet microscopy along several hundred micrometers of the zebrafish spinal cord. The recordings show the strikingly stereotyped spatio-temporal control that governs midline crossing. Commissural axons are observed crossing the ventral floor plate midline perpendicularly at about 20 microns/h, in a manner dependent on the Robo3 receptor and with a growth rate minimum around the… Expand

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