Corpus ID: 235794893

Real time large scale $\textit{in vivo}$ observations by light-sheet microscopy reveal intrinsic synchrony, plasticity and growth cone dynamics of midline crossing axons at the ventral floor plate of the zebrafish spinal cord

  title={Real time large scale \$\textit\{in vivo\}\$ observations by light-sheet microscopy reveal intrinsic synchrony, plasticity and growth cone dynamics of midline crossing axons at the ventral floor plate of the zebrafish spinal cord},
  author={S{\o}ren S L Andersen},
Axonal growth and guidance at the ventral floor plate is here followed in vivo in real time at high resolution by light-sheet microscopy along several hundred micrometers of the zebrafish spinal cord. The recordings show the strikingly stereotyped spatio-temporal control that governs midline crossing. Commissural axons are observed crossing the ventral floor plate midline perpendicularly at about 20 microns/h, in a manner dependent on the Robo3 receptor and with a growth rate minimum around the… Expand


Pathfinding by growth cones of commissural interneurons in the chick embryo spinal cord: A light and electron microscopic study
Electron microscopic observations revealed that late developing growth cones always contacted or fasciculated with preexisting axons and that the cellular environment through which they grow is oriented in such a way that the growth cones appear to be guided in specific directions. Expand
Growth cone dynamics during the migration of an identified commissural growth cone
  • P. Z. Myers, M. Bastiani
  • Biology, Medicine
  • The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1993
Time-lapse video microscopy is used to study the behavior of a neuron, Q1, that pioneers the posterior commissure of the embryonic grasshopper to identify stereotypic activities that might indicate important interactions necessary for proper formation of the commissures. Expand
Guidance of commissural growth cones at the floor plate in embryonic rat spinal cord.
The reorientation of axons in register with the floor plate boundary and the change in the morphological properties of commissural growth cones as they traverse the midline suggest that the floor Plate may act as a guidepost with functions similar to cells that have been implicated in axon guidance in invertebrates. Expand
Growth Cone Form Is Behavior-Specific and, Consequently, Position-Specific along the Retinal Axon Pathway
Video time-lapse microscopy has made it possible to document growth cone motility during axon navigation in the intact brain, and growth cone form appears to be position-specific in static preparations, due to the fraction of time spent in a given state in different locales. Expand
Axon fasciculation and differences in midline kinetics between pioneer and follower axons within commissural fascicles
This work follows forebrain commissure formation in living zebrafish embryos using timelapse fluorescence microscopy to examine quantitatively commissural axon kinetics at the midline: a place where axon interactions might be important. Expand
Midline crossing is not required for subsequent pathfinding decisions in commissural neurons
It is demonstrated that midline Crossing is not required for subsequent guidance decisions by pioneer axons and that this is due, in part, to DCC inhibition of Robo2 function prior to midline crossing. Expand
Ipsi‐ and contralateral commissural growth cones react differently to the cellular environment of the ventral zebrafish spinal cord
  • R. Bernhardt
  • Biology, Medicine
  • The Journal of comparative neurology
  • 1994
A change occurs in the response characteristics of commissural growth cones as they cross the ventral midline of the spinal cord, which could help to explain why the growth cones extend first toward but then away from the ventrally midline. Expand
A confocal study of spinal interneurons in living larval zebrafish
Four newly discovered cell types are described here for the first time, including classes of commissural neurons with axons that ascend, descend, and bifurcate in the contralateral spinal cord of larval zebrafish, leading to predictions of the roles of some of these interneurons in motor circuits. Expand
Pioneer midbrain longitudinal axons navigate using a balance of Netrin attraction and Slit repulsion
Evidence suggests that longitudinal axons respond directly and simultaneously to both attractants and repellents, and that the combined signals constrain axons to grow longitudinally. Expand
Whole-brain serial-section electron microscopy in larval zebrafish
This work presents ssEM data for the complete brain of a larval zebrafish at 5.5 days post-fertilization to reduce acquisition time and data management requirements and set the stage for whole-brain structure–function comparisons by co-registering functional reference atlases and in vivo two-photon fluorescence microscopy data from the same specimen. Expand