Real-time in vivo bioluminescence imaging of lentiviral vector-mediated gene transfer in mouse testis.

@article{Kim2010RealtimeIV,
  title={Real-time in vivo bioluminescence imaging of lentiviral vector-mediated gene transfer in mouse testis.},
  author={Tae-shin Kim and H Steven Choi and Byoung Y. Ryu and G T Gang and Sun Uk Kim and Deog-Bon Koo and Jin-man Kim and Jessica H. Han and C. K. Park and Song Her and Dong Seok Lee},
  journal={Theriogenology},
  year={2010},
  volume={73 1},
  pages={129-38}
}
Although much research has focused on transferring exogenous genes into living mouse testis to investigate specific gene functions in spermatogenic, Sertoli, and Leydig cells, relatively little is known regarding real-time gene expression in vivo. In this study, we constructed a bicistronic lentiviral vector (LV) encoding firefly luciferase and enhanced green fluorescence protein (EGFP); this was a highly efficient in vivo gene transfer tool. After microinjecting LV into the seminiferous… CONTINUE READING

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  • G Villuendas, A Gutierrez-Adan, A Jimenez, C Rojo, ER Roldan, B Pintado
  • 2010

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