Real-time in vivo bioluminescence imaging of lentiviral vector-mediated gene transfer in mouse testis.

@article{Kim2010RealtimeIV,
  title={Real-time in vivo bioluminescence imaging of lentiviral vector-mediated gene transfer in mouse testis.},
  author={T. Kim and H. Choi and B. Ryu and G. Gang and S. U. Kim and D. B. Koo and J. Kim and J. Han and C. Park and S. Her and D. Lee},
  journal={Theriogenology},
  year={2010},
  volume={73 1},
  pages={
          129-38
        }
}
  • T. Kim, H. Choi, +8 authors D. Lee
  • Published 2010
  • Biology, Medicine
  • Theriogenology
  • Although much research has focused on transferring exogenous genes into living mouse testis to investigate specific gene functions in spermatogenic, Sertoli, and Leydig cells, relatively little is known regarding real-time gene expression in vivo. In this study, we constructed a bicistronic lentiviral vector (LV) encoding firefly luciferase and enhanced green fluorescence protein (EGFP); this was a highly efficient in vivo gene transfer tool. After microinjecting LV into the seminiferous… CONTINUE READING
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