Real-time imaging of tumor-cell shedding and trafficking in lymphatic channels.

@article{Hayashi2007RealtimeIO,
  title={Real-time imaging of tumor-cell shedding and trafficking in lymphatic channels.},
  author={Katsuhiro Hayashi and Ping Jiang and Kensuke Yamauchi and Norio Yamamoto and Hiroyuki Tsuchiya and K Tomita and Abdool R. Moossa and Michael Bouvet and Robert M Hoffman},
  journal={Cancer research},
  year={2007},
  volume={67 17},
  pages={8223-8}
}
In the present report, we show real-time imaging of cancer cell trafficking in lymphatic vessels. Cancer cells labeled with both green fluorescent protein (GFP) in the nucleus and red fluorescent protein (RFP) in the cytoplasm or with GFP only or RFP only were injected into the inguinal lymph node of nude mice. The labeled cancer cells trafficked through lymphatic vessels where they were imaged via a skin flap in real time at the cellular level until they entered the axillary lymph node. The… CONTINUE READING
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