Real-time imaging of neurons retrogradely and anterogradely labelled with calcium-sensitive dyes

  title={Real-time imaging of neurons retrogradely and anterogradely labelled with calcium-sensitive dyes},
  author={Michael J. O'Donovan and Stephen M. Ho and Gerald N. Sholomenko and W Yee},
  journal={Journal of Neuroscience Methods},

Calcium imaging of rhythmic network activity in the developing spinal cord of the chick embryo

Video-rate imaging of spinal neurons loaded with calcium-sensitive dyes was used to investigate the calcium dynamics and cellular organization of spontaneously active rhythm-generating networks in the spinal cord of E9-E12 chick embryos, suggesting that neurons in the ventrolateral part of the spinal Cord are important for rhythmogenesis and that axons traveling in the Ventrolateral white matter may be involved in the rhythmic excitation of motoneurons and interneurons.

Imaging Brain Activity With Voltage- and Calcium-Sensitive Dyes

This paper presents three examples of imaging brain activity with voltage- or calcium-sensitive dyes and then discusses the methodological aspects of the measurements that are needed to achieve an

Electroporation loading of calcium-sensitive dyes into the CNS.

The use of electroporation to load neurons with calcium-sensitive dyes in the isolated spinal cord of the neonatal mouse in vitro is described and it is shown that these different motor behaviors are characterized by distinct patterns of activation among the labeled populations of cells.

Calcium Imaging for Detection and Estimation of Spike Activities in Aplysia Neurons

Abstract We explored whether the calcium imaging can be applicable to detection of spike activity of Aplysia wide-spread neurons. Well-used procedures, the membrane permeable acetoxymethyl (AM) types



Mapping calcium transients in the dendrites of Purkinje cells from the guinea‐pig cerebellum in vitro.

The results indicate that the absorbance signals came from calcium entry into the cell resulting from the turning on of voltage‐dependent calcium conductances, and suggests that calcium spikes causing these signals can be evoked separately in different regions of the Purkinje cell dendritic field by long‐lasting potentials which may reach local threshold at different times.

Fluorescent carbocyanine dyes allow living neurons of identified origin to be studied in long-term cultures

The results indicate that preganglionic neurons can survive in the absence of their target cells and that several aspects of their differentiation in the presence of target appear normal.

The development of sensorimotor synaptic connections in the lumbosacral cord of the chick embryo

In the chick, as in other species, a glutamate-like transmitter appears to be released at the synapses between muscle afferents and motoneurons, and the EPSP in these young embryos was unaffected by picrotoxin and strychnine, but responded to APV and kynurenate in a manner similar to that at later stages.

Development of spinal motor networks in the chick embryo.

The findings suggest that functional descending connections from the brainstem to the lumbar cord are present by E7, although activation of ascending axons or electrical synapses cannot be eliminated.

Subcellular calcium transients visualized by confocal microscopy in a voltage-clamped vertebrate neuron.

Confocal laser-scanned microscopy and long-wavelength calcium (Ca2+) indicators were combined to monitor both sustained and rapidly dissipating Ca2+ gradients in voltage-clamped sympathetic neurons isolated from the bullfrog, suggesting an amplification mechanism involving Ca2(+)-induced Ca2+.