Real-time imaging of neurons retrogradely and anterogradely labelled with calcium-sensitive dyes

@article{ODonovan1993RealtimeIO,
  title={Real-time imaging of neurons retrogradely and anterogradely labelled with calcium-sensitive dyes},
  author={Michael J. O'Donovan and S. Ho and G. Sholomenko and W. Yee},
  journal={Journal of Neuroscience Methods},
  year={1993},
  volume={46},
  pages={91-106}
}
Membrane-impermeant calcium indicator dyes were used to retrogradely label dorsal root ganglia, spinal motoneurons and interneurons in the spinal cord of the chick embryo. The dyes were also used to label anterogradely primary afferent axons in the spinal cord and synaptic endings in the ciliary ganglion. Labelled neurons were imaged using digital videomicroscopy. Motoneurons and dorsal root ganglion cells exhibited a frequency-dependent change in fluorescence during antidromic stimulation… Expand
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