Real-time imaging of intracellular calcium change with simultaneous single channel recording in mammary epithelial cells.

@article{Furuya1990RealtimeIO,
  title={Real-time imaging of intracellular calcium change with simultaneous single channel recording in mammary epithelial cells.},
  author={Kishio Furuya and Koh-ichi Enomoto},
  journal={Brain research bulletin},
  year={1990},
  volume={25 5},
  pages={779-81}
}
A method of continuous image subtraction of fura-2 fluorescence made it possible to observe real-time (video rate) changes in intracellular calcium (Cai). This simple method is very useful for simultaneous measurements in electrophysiology with a system containing cells of different states. A method for synchronous recording of Cai change and single channel activity is also described. In cultured mammary epithelial cells, these methods revealed a propagating Cai signal induced by mechanical… CONTINUE READING

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