Real-time imaging of glucocorticoid receptor dynamics in living neurons and glial cells in comparison with non-neural cells.

@article{Nishi1999RealtimeIO,
  title={Real-time imaging of glucocorticoid receptor dynamics in living neurons and glial cells in comparison with non-neural cells.},
  author={Mayumi Nishi and Naomi Takenaka and Noriyuki Morita and Takao Ito and Hitoshi Ozawa and Mitsuhiro Kawata},
  journal={The European journal of neuroscience},
  year={1999},
  volume={11 6},
  pages={
          1927-36
        }
}
To investigate the intracellular trafficking of glucocorticoid receptor (GR) in response to various conditions in a single living cell, a green fluorescent protein (GFP) and rat GR chimera construct (GFP-GR) was prepared. We transiently transfected GFP-GR into primary cultured rat hippocampal neurons, cortical glial cells, and non-neural cells, e.g. COS-1 cells and CV-1 cells, and compared the dynamic changes in subcellular localization of GFP-GR in these cells. When GFP-GR was expressed in the… CONTINUE READING
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