Real-time gene expression analysis in carp (Cyprinus carpio L.) skin: inflammatory responses caused by the ectoparasite Ichthyophthirius multifiliis.

@article{Gonzalez2007RealtimeGE,
  title={Real-time gene expression analysis in carp (Cyprinus carpio L.) skin: inflammatory responses caused by the ectoparasite Ichthyophthirius multifiliis.},
  author={Santiago Fernandez Gonzalez and Kurt Buchmann and Michael Canova Engelbrecht Nielsen},
  journal={Fish & shellfish immunology},
  year={2007},
  volume={22 6},
  pages={
          641-50
        }
}
Real time quantitative PCR (RQ-PCR) assays were developed for the measurement of differential real-time expression of immune-related genes in skin and whole blood from Cyprinus carpio during an infection with the ectoparasite Ichthyophthirius multifiliis. The target genes included the chemokines CXCa and CXCb, the chemokine receptors CXCR1 and CXCR2, the pro-inflammatory cytokines interleukin 1 beta (IL-1beta) and tumour necrosis factor alpha (TNF-alpha) and the enzymes inducible nitric oxide… CONTINUE READING
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