Real-time fluorescence PCR assays for detection and characterization of heat-labile I and heat-stable I enterotoxin genes from enterotoxigenic Escherichia coli.

@article{Reischl2004RealtimeFP,
  title={Real-time fluorescence PCR assays for detection and characterization of heat-labile I and heat-stable I enterotoxin genes from enterotoxigenic Escherichia coli.},
  author={Udo Reischl and Mohammad T. Youssef and Hans Wolf and Eija K Hyytia-Trees and Nancy A. Strockbine},
  journal={Journal of clinical microbiology},
  year={2004},
  volume={42 9},
  pages={4092-100}
}
To facilitate the diagnosis of enterotoxigenic Escherichia coli (ETEC) infections in humans, we developed and evaluated real-time fluorescence PCR assays for the Roche LightCycler (LC) against the enterotoxin genes commonly present in strains associated with human illness. Separate LC-PCR assays with identical cycling conditions were designed for the type I heat-labile enterotoxin (LT I) and the type I heat-stable enterotoxin (ST I) genes, using the LC hybridization probe format. A duplex assay… CONTINUE READING
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