Real-time detection and quantification of telomerase activity utilizing energy transfer primers.

@article{Uehara2006RealtimeDA,
  title={Real-time detection and quantification of telomerase activity utilizing energy transfer primers.},
  author={Hiroshi Uehara},
  journal={Methods in molecular biology},
  year={2006},
  volume={335},
  pages={157-69}
}
A novel closed-tube format telomeric repeat amplification protocol specifically adapted to real-time detection and quantification of telomerase activity was developed. The assay utilizes energy transfer primers, which emit fluorescence only upon incorporation into polymerase chain reaction (PCR) amplification products. The assay, performed on a real-time detection instrument, is highly reproducible, sensitive, and specific. Telomerase activity in as few as 10 cultured cells can be quantified… CONTINUE READING

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