Real-time cdPCR opens a window into events occurring in the first few PCR amplification cycles.

Abstract

Polymerase chain reaction (PCR) end-point limiting dilution techniques, collectively termed "digital PCR (dPCR)", have been proposed as providing a potentially primary method for DNA quantification. We are evaluating several commercially available dPCR systems for use in certifying mass concentration in human genomic DNA reference materials. To better… (More)
DOI: 10.1007/s00216-015-9073-8

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