Real-time calcium response of cultured bone cells to fluid flow.

  title={Real-time calcium response of cultured bone cells to fluid flow.},
  author={Clark Tung-Hui Hung and Solomon R. Pollack and Todd Reilly and Carl T. Brighton},
  journal={Clinical orthopaedics and related research},
Using a parallel-plate flow chamber and fura-2 fluorescence microscopy, intracellular calcium was measured cell by cell in preconfluent primary culture rat calvarial bone cells to 18, 35, and 70 dynes/cm2 of fluid-induced shear stress. A heterogeneous response with respect to peak amplitude and latency was observed for the culture, with an overriding dose-dependent relationship between the mean peak amplitude of response and shear-stress magnitude. A dose dependence was observed between the… CONTINUE READING

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