Real-time SNP analysis in secondary-structure-folded nucleic acids.

Abstract

Hybridization of two complementary nucleic acid strands is extensively used in the analysis of specific DNA/RNA sequences in real-time PCR, DNA microarrays, and techniques for RNA monitoring in living cells. The design of the hybridization probes is based on A–T and G–C complementarity and may seem straightforward. However, singlestranded DNA and RNA… (More)
DOI: 10.1002/anie.201004475

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