Real-time PCR with serum samples is indispensable for early diagnosis of acute Q fever.

@article{Schneeberger2010RealtimePW,
  title={Real-time PCR with serum samples is indispensable for early diagnosis of acute Q fever.},
  author={Peter Martin Schneeberger and Mirjam H. A. Hermans and Erik J. van Hannen and Jeroen J A Schellekens and Alexander C. A. P. Leenders and Peter Cornelis Wever},
  journal={Clinical and vaccine immunology : CVI},
  year={2010},
  volume={17 2},
  pages={286-90}
}
The world's largest Q fever outbreak is ongoing in The Netherlands with around 3,000 confirmed cases since the first half of 2007. Increased awareness has resulted in early referral of patients for diagnostics. An important drawback to serological diagnosis of acute Q fever is the lag phase in antibody response. Therefore, we evaluated the performance of a real-time PCR for detection of Coxiella burnetii DNA using serum samples from patients with acute Q fever. PCR, targeting IS1111, was… CONTINUE READING