Real-time PCR targeting a 529-bp repeat element for diagnosis of toxoplasmosis.

  title={Real-time PCR targeting a 529-bp repeat element for diagnosis of toxoplasmosis.},
  author={Benjamin Edvinsson and Maija Lappalainen and Birgitta Eveng{\aa}rd},
  journal={Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases},
  volume={12 2},
Sensitive and rapid detection of infection with Toxoplasma gondii in transplanted immunocompromised patients is crucial for a good prognosis. Two DNA fragments are used currently for detecting T. gondii infection by PCR, i.e., the B1 gene and a 529-bp repeat element that exists in 200-300 copies/genome. This study investigated whether targeting the 529-bp repeat element gives better sensitivity and accuracy than can be obtained when targeting the B1 gene (35 copies) when concentrations of T… CONTINUE READING

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