Real-time PCR system for detection of orthopoxviruses and simultaneous identification of smallpox virus.

@article{Olson2004RealtimePS,
  title={Real-time PCR system for detection of orthopoxviruses and simultaneous identification of smallpox virus.},
  author={Victoria A. Olson and Thomas Peter Laue and Miriam T Laker and Igor V. Babkin and Christian Drosten and Sergei Nikolaevich Shchelkunov and Matthias Niedrig and Inger K. Damon and Hermann von Meyer},
  journal={Journal of clinical microbiology},
  year={2004},
  volume={42 5},
  pages={
          1940-6
        }
}
A screening assay for real-time LightCycler (Roche Applied Science, Mannheim, Germany) PCR identification of smallpox virus DNA was developed and compiled in a kit system under good manufacturing practice conditions with standardized reagents. In search of a sequence region unique to smallpox virus, the nucleotide sequence of the 14-kDa fusion protein gene of each of 14 variola virus isolates of the Russian World Health Organization smallpox virus repository was determined and compared to… CONTINUE READING
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