Real-time PCR quantification of bacterial adhesion to Caco-2 cells: competition between bifidobacteria and enteropathogens.

@article{Candela2005RealtimePQ,
  title={Real-time PCR quantification of bacterial adhesion to Caco-2 cells: competition between bifidobacteria and enteropathogens.},
  author={Marco Candela and Gerd M Seibold and Beatrice Vitali and Sabrina M Lachenmaier and Bernhard J Eikmanns and Patrizia Brigidi},
  journal={Research in microbiology},
  year={2005},
  volume={156 8},
  pages={887-95}
}
Probiotic bacteria play an important role in protecting the host from intestinal colonization of pathogenic bacteria. We have developed a new analytical approach based on a real-time PCR technique for quantifying Bifidobacterium adhesion to intestinal epithelial cells. Real-time PCR analysis showed that adhesion to enterocyte-like Caco-2 cells represented a variable phenotype in the genus Bifidobacterium, enabling classification of three adhesion behaviors: high adhesiveness (>40… CONTINUE READING

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