Real-time PCR quantification of Dehalococcoides populations: methods and applications.

@article{Cupples2008RealtimePQ,
  title={Real-time PCR quantification of Dehalococcoides populations: methods and applications.},
  author={Alison M Cupples},
  journal={Journal of microbiological methods},
  year={2008},
  volume={72 1},
  pages={
          1-11
        }
}
Dehalococcoides species are responsible for the reductive dehalogenation of an impressive range of common, persistent environmental contaminants. These microorganisms are difficult to both obtain and grow in pure culture, and so are often studied while they exist in consortia using molecular techniques. In particular, a significant number of quantitative real-time PCR (qPCR) assays targeting Dehalococcoides spp. have been reported. Initial qPCR methods targeted the 16S rRNA gene, however… CONTINUE READING
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