Real-time PCR quantification and live-cell imaging of endophytic colonization of barley (Hordeum vulgare) roots by Fusarium equiseti and Pochonia chlamydosporia.

@article{MaciVicente2009RealtimePQ,
  title={Real-time PCR quantification and live-cell imaging of endophytic colonization of barley (Hordeum vulgare) roots by Fusarium equiseti and Pochonia chlamydosporia.},
  author={Jose G Maci{\'a}-Vicente and H. -B. Jansson and Nicholas J. Talbot and Luis Vicente L{\'o}pez-Llorca},
  journal={The New phytologist},
  year={2009},
  volume={182 1},
  pages={213-28}
}
*New tools were developed for the study of the endophytic development of the fungal species Fusarium equiseti and Pochonia chlamydosporia in barley (Hordeum vulgare) roots. These were applied to monitor the host colonization patterns of these potential candidates for biocontrol of root pathogens. * Molecular beacons specific for either F. equiseti or P. chlamydosporia were designed and used in real-time polymerase chain reaction (PCR) quantification of fungal populations in roots. Genetic… CONTINUE READING