Real-time PCR for the detection of Giardia lamblia.

  title={Real-time PCR for the detection of Giardia lamblia.},
  author={Jaco J. Verweij and Janke Schinkel and Daphne Laeijendecker and Marianne A A van Rooyen and Lisette van Lieshout and Anton M. Polderman},
  journal={Molecular and cellular probes},
  volume={17 5},
Microscopy is considered to be the gold standard for diagnosis of Giardia lamblia infection. However, this method is time-consuming and not very sensitive. We developed a real-time PCR assay based on the small subunit ribosomal RNA gene of G. lamblia for the specific detection of G. lamblia DNA in stool samples and thereafter compared the results with microscopy and antigen detection. The G. lamblia real-time PCR was positive in 102 of 104 fecal samples known to contain G. lamblia cysts and was… CONTINUE READING

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