Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridization probes.

@article{Costa2000RealtimePF,
  title={Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridization probes.},
  author={Jos{\'e} Manuel Da Costa and C{\'e}cile Pautas and Pauline Ernault and Françoise Foulet and Catherine Cordonnier and St{\'e}phane Bretagne},
  journal={Journal of clinical microbiology},
  year={2000},
  volume={38 8},
  pages={
          2929-32
        }
}
Toxoplasma reactivation is a life-threatening complication of allogeneic stem cell transplantation. A poor prognosis is probably linked to a difficult diagnosis, based on the detection of evidence of parasites in tissue. We developed a real-time PCR test using fluorescence resonance energy transfer hybridization probes to detect and quantify Toxoplasma gondii DNA in serum. This PCR test gave reproducible quantitative results over a dynamic range of from 0.75 x 10(6) to 0.75 parasites per PCR… CONTINUE READING

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