Real-time PCR detection of host-mediated cyanophage gene transcripts during infection of a natural Microcystis aeruginosa population.

@article{Yoshida2010RealtimePD,
  title={Real-time PCR detection of host-mediated cyanophage gene transcripts during infection of a natural Microcystis aeruginosa population.},
  author={Mitsuhiro Yoshida and Takashi Yoshida and Yukari Yoshida-Takashima and Aki Kashima and Shingo Hiroishi},
  journal={Microbes and environments},
  year={2010},
  volume={25 3},
  pages={211-5}
}
The aim of this study was to develop a quantitative real-time reverse transcription-PCR (real-time RT-PCR) assay to detect and quantify mRNA of cyanophages within infected Microcystis aeruginosa cells in a freshwater pond. Laboratory-based data showed that the relative abundance of the cyanophage g91 mRNA within host cells increased before cyanophage numbers increased in culture. This transcriptional pattern indicated the kinetics of the viral infection suggesting the real-time RT-PCR method to… CONTINUE READING

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