Real-time PCR assay using molecular beacon for quantitation of hepatitis B virus DNA.

@article{Sum2004RealtimePA,
  title={Real-time PCR assay using molecular beacon for quantitation of hepatitis B virus DNA.},
  author={Simon Siu-Man Sum and Danny Ka-ho Wong and Man-fung Yuen and He-Jun Yuan and Jian Yu and Ching-lung Lai and David Ho and Linqi Zhang},
  journal={Journal of clinical microbiology},
  year={2004},
  volume={42 8},
  pages={3438-40}
}
Levels of hepatitis B virus (HBV) DNA in the blood serve as an important marker in monitoring the disease progression and treatment efficacy of chronic HBV infection. Several commercial assays are available for accurate measurement of HBV genomic DNA, but many of them are hampered by relatively low sensitivity and limited dynamic range. The aim of this study was to develop a sensitive and accurate assay for measuring HBV genomic DNA using real-time PCR with a molecular beacon (HBV beacon assay… CONTINUE READING

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