Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii.

Abstract

Detection of Bordetella holmesii by a real-time PCR assay targeting IS481 of Bordetella pertussis is reported. Sequencing of IS481-specific PCR products from B. pertussis and B. holmesii isolates revealed sequence homology. Restriction fragment length polymorphism demonstrated a low copy number of IS481-like sequences in B. holmesii. These results, and… (More)

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Cite this paper

@article{Reischl2001RealtimePA, title={Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii.}, author={Udo Reischl and Norbert Lehn and Gary N. Sanden and Michael J. Loeffelholz}, journal={Journal of clinical microbiology}, year={2001}, volume={39 5}, pages={1963-6} }