Real-time PCR assay for rapid and accurate detection of point mutations conferring resistance to clarithromycin in Helicobacter pylori.

@article{Oleastro2003RealtimePA,
  title={Real-time PCR assay for rapid and accurate detection of point mutations conferring resistance to clarithromycin in Helicobacter pylori.},
  author={M{\'o}nica Oleastro and Armelle M{\'e}nard and Adriana L dos Santos and H{\'e}rve Lamouliatte and Lurdes Monteiro and Philippe Barth{\'e}l{\'e}my and Francis M{\'e}graud},
  journal={Journal of clinical microbiology},
  year={2003},
  volume={41 1},
  pages={397-402}
}
The main cause of failure of Helicobacter pylori eradication therapy is resistance to clarithromycin. The resistance is due to three point mutations in two positions on the 23S rRNA (A2142C, A2142G, and A2143G). Our aim was to develop a rapid and accurate method to detect these mutations directly on biopsy specimens. We developed a real-time PCR that included a simultaneous detection of the amplicons by hybridization of two probes labeled with LC-Red and fluorescein by using the fluorescence… CONTINUE READING
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