Real-time PCR assay for measurement of mouse telomeres.

  title={Real-time PCR assay for measurement of mouse telomeres.},
  author={Ralph J Callicott and James E. Womack},
  journal={Comparative medicine},
  volume={56 1},
Measurement of telomeres by polymerase chain reaction (PCR) amplification has been problematic due to the formation of dimers by the primers designed to hybridize to the telomere repeats. Recently, a set of primers that overcome this problem has been created and used to develop an assay to measure human telomeres by real-time quantitative PCR. We modified this assay to measure mouse telomeres. Results showed that the primers do indeed amplify mammalian telomere repeats without forming dimers… CONTINUE READING