Real-time PCR assay for identification of Blastomyces dermatitidis in culture and in tissue.


The TaqMan real-time PCR assay was developed from the Blastomyces dermatitidis BAD1 gene promoter. The assay identified all haplotypes of B. dermatitidis and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast form of B. dermatitidis. No cross-reactivity was observed… (More)
DOI: 10.1128/JCM.00310-12


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