Real-time PCR array chip with capillary-driven sample loading and reactor sealing for point-of-care applications.

@article{Ramalingam2009RealtimePA,
  title={Real-time PCR array chip with capillary-driven sample loading and reactor sealing for point-of-care applications.},
  author={Naveen Ramalingam and Hao-Bing Liu and Chang-Chun Dai and Yu Jiang and Hui Wang and Qinghui Wang and Kam M Hui and H. Gong},
  journal={Biomedical microdevices},
  year={2009},
  volume={11 5},
  pages={1007-20}
}
A major challenge for the lab-on-a-chip (LOC) community is to develop point-of-care diagnostic chips that do not use instruments. Such instruments include pumping or liquid handling devices for distribution of patient's nucleic-acid test sample among an array of reactors and microvalves or mechanical parts to seal these reactors. In this paper, we report the development of a primer pair pre-loaded PCR array chip, in which the loading of the PCR mixture into an array of reactors and subsequent… CONTINUE READING