Real-time PCR: duplexing without optimization.

  title={Real-time PCR: duplexing without optimization.},
  author={Andreas Edgar Hein and Ursula Bodendorf},
  journal={Analytical biochemistry},
  volume={360 1},
Real-time PCR applications generally require determination of the threshold cycle of a gene of interest in parallel with an endogenous control. In standard situations, the gene-specific and control reactions are run as separate samples (singleplex). In contrast, duplex approaches combine both reactions within a single well, thereby saving time, cost, and material. However, establishing duplex reactions usually requires laborious optimization justifiable only for the analysis of large sample… CONTINUE READING
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