Real-time NASBA for Detection of Strawberry Vein Banding Virus

  title={Real-time NASBA for Detection of Strawberry Vein Banding Virus},
  author={D. Hanzlikov{\'a} Va{\vs}kov{\'a} and Josef {\vS}pak and Michel M Klerks and Cor D. Schoen and John R. Thompson and Wilhelm Jelkmann},
  journal={European Journal of Plant Pathology},
An assay for the detection of Strawberry vein banding virus (SVBV) in Fragaria spp. based on nucleic acid sequence based amplification (NASBA) and real-time detection using molecular beacons (real-time NASBA) is described. This assay was compared both with biological indexing, the current method for certification of SVBV, and a newly optimised PCR-based detection method. Performance of the assay was tested on three SVBV isolates in Fragaria indicator plants and 317 field strawberries from five… CONTINUE READING


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