Real-time DNA quantification of nuclear and mitochondrial DNA in forensic analysis.

@article{Andrasson2002RealtimeDQ,
  title={Real-time DNA quantification of nuclear and mitochondrial DNA in forensic analysis.},
  author={Hanna Andr{\'e}asson and Ulf Gyllensten and Marie Allen},
  journal={BioTechniques},
  year={2002},
  volume={33 2},
  pages={402-4, 407-11}
}
The rapid development of molecular genetic analysis tools has made it possible to analyze most biological materialfound at the scene of a crime. Evidence materials containing DNA quantities too low to be analyzed using nuclear markers can be analyzed using the highly abundant mtDNA. However, there is a shortage of sensitive nDNA and mtDNA quantification assays. In this study, an assay for the quantification of very small amounts of DNA, based on the real-time Taq-Man assay, has been developed… CONTINUE READING