Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences

@article{Bustin2005RealtimeFQ,
  title={Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences},
  author={S. Bustin},
  journal={Expert Review of Molecular Diagnostics},
  year={2005},
  volume={5},
  pages={493 - 498}
}
  • S. Bustin
  • Published 2005
  • Biology, Medicine
  • Expert Review of Molecular Diagnostics
Real-time fluorescence-based quantitative PCR has become established as the benchmark technology for the quantification of nucleic acids, offering an immense choice of protocols, chemistries and instruments. However, whilst there are comparatively few technical problems associated with DNA-targeted quantitative PCR, this is not the case for real-time reverse transcription PCR assays, and there is considerable uncertainty regarding biological or clinical relevance of many real-time reverse… Expand
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References

SHOWING 1-10 OF 47 REFERENCES
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.
  • S. Bustin
  • Computer Science, Medicine
  • Journal of molecular endocrinology
  • 2002
  • 2,298
  • PDF
Real-time PCR in the microbiology laboratory.
  • I. Mackay
  • Biology, Medicine
  • Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
  • 2004
  • 676
  • PDF
Real-time PCR in virology.
  • 1,177
  • PDF
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.
  • S. A. Bustin
  • Biology, Medicine
  • Journal of molecular endocrinology
  • 2000
  • 3,728
  • PDF
Real-time RT-PCR normalisation; strategies and considerations
  • 1,589
  • PDF
Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream.
  • 1,239
  • PDF
Quantitative real-time RT-PCR--a perspective.
  • 1,097
  • PDF
Quantification using real-time PCR technology: applications and limitations.
  • D. Klein
  • Medicine, Biology
  • Trends in molecular medicine
  • 2002
  • 633
  • PDF
A new mathematical model for relative quantification in real-time RT-PCR.
  • M. Pfaffl
  • Biology, Medicine
  • Nucleic acids research
  • 2001
  • 26,312
  • PDF
Improved quantitative real-time RT-PCR for expression profiling of individual cells.
  • B. Liss
  • Biology, Medicine
  • Nucleic acids research
  • 2002
  • 146
  • PDF
...
1
2
3
4
5
...