Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences

@article{Bustin2005RealtimeFQ,
  title={Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences},
  author={S. Bustin},
  journal={Expert Review of Molecular Diagnostics},
  year={2005},
  volume={5},
  pages={493 - 498}
}
  • S. Bustin
  • Published 2005
  • Biology, Medicine
  • Expert Review of Molecular Diagnostics
Real-time fluorescence-based quantitative PCR has become established as the benchmark technology for the quantification of nucleic acids, offering an immense choice of protocols, chemistries and instruments. However, whilst there are comparatively few technical problems associated with DNA-targeted quantitative PCR, this is not the case for real-time reverse transcription PCR assays, and there is considerable uncertainty regarding biological or clinical relevance of many real-time reverse… Expand
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