Real-Time RT-PCR assay to quantify the expression of fum1 and fum19 genes from the Fumonisin-producing Fusarium verticillioides.

@article{LpezErrasqun2007RealTimeRA,
  title={Real-Time RT-PCR assay to quantify the expression of fum1 and fum19 genes from the Fumonisin-producing Fusarium verticillioides.},
  author={Elena L{\'o}pez-Errasqu{\'i}n and Covadonga Vazquez and Misericordia Jim{\'e}nez and Mar{\'i}a Teresa Gonz{\'a}lez-Ja{\'e}n},
  journal={Journal of microbiological methods},
  year={2007},
  volume={68 2},
  pages={312-7}
}
Fumonisins are a group of mycotoxins produced by Fusarium species of the Gibberella fujikuroi species complex that contaminate food and feed products, and represent a risk for human and animal health. In this work, we have developed a specific real-time reverse transcription-PCR (RT-PCR) assay to quantify the level of expression of two genes of the fumonisin biosynthetic cluster in F. verticillioides: fum1 (that encodes a polyketide synthase enzyme) and the ABC transporter encoding gene fum19… CONTINUE READING
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