Real-Time PCR in HIV/Trypanosoma cruzi Coinfection with and without Chagas Disease Reactivation: Association with HIV Viral Load and CD4+ Level

@inproceedings{Freitas2011RealTimePI,
  title={Real-Time PCR in HIV/Trypanosoma cruzi Coinfection with and without Chagas Disease Reactivation: Association with HIV Viral Load and CD4+ Level},
  author={Vera L{\'u}cia Teixeira de Freitas and Sheila Cristina Vicente da Silva and Ana Marli Christovam Sartori and Rita Cristina Bezerra and Elizabeth Visone Nunes Westphalen and Tatiane Decaris Molina and Antonio R. L. Teixeira and Karim Ibrahim and Maria Aparecida Shikanai-Yasuda},
  booktitle={PLoS neglected tropical diseases},
  year={2011}
}
BACKGROUND Reactivation of chronic Chagas disease, which occurs in approximately 20% of patients coinfected with HIV/Trypanosoma cruzi (T. cruzi), is commonly characterized by severe meningoencephalitis and myocarditis. The use of quantitative molecular tests to monitor Chagas disease reactivation was analyzed. METHODOLOGY Polymerase chain reaction (PCR) of kDNA sequences, competitive (C-) PCR and real-time quantitative (q) PCR were compared with blood cultures and xenodiagnosis in samples… CONTINUE READING
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